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List the similarities and differences between aerobic and anaerobic mode of respiration Important

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Give an experiment to prove that carbon dioxide is released during respiration. You are here

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Class 10
Chapter 6 Class 10 - Life Processes

Give an experiment to prove that carbon dioxide is released during respiration.

 

Answer:

Respiration is a biochemical process in which the food is oxidised in order to release energy.

To prove that CO 2 is released during respiration, 

  • We take germinating seeds which are actively respiring to produce CO 2 a
  • And we use Potassium Hydroxide (KOH) to absorb this released CO 2 .
    The overall reaction is as follows:  

Test to confirm presence of CO2 - Teachoo.jpg

Materials Required:

Conical flask, U-shaped delivery tube (tube bent twice at right angles), cotton wool or moist blotting paper, water, thread, beaker, test tube, rubber cork with one hole, 20% freshly prepared KOH solution, vaseline, soaked gram seeds.

Procedure:

  1. Take about 25-30 seeds of gram and germinate these seeds by placing them on moist cotton wool or moist blotting paper for 3-4 days.
  2. Place the germinated seeds into a conical flask and sprinkle a little water in the flask to moist the seeds.
  3. Take freshly prepared 20% KOH solution in a test tube and hang it in a conical flask with help of thread.
  4. Close the mouth of the conical flask by placing a rubber cork containing one hole. .
  5. Through the hole of rubber cork, insert one end of the U-shaped glass delivery tube in the conical flask and place the other end into a beaker filled with water.
  6. Seal all the connections of the experimental set-up with vaseline so as to make it air-tight.
  7. Mark the initial level of water in the U-shaped delivery tube.
  8. Keep the apparatus undisturbed for 1-2 hours and note the change in level of water in the delivery tube.

Observation :

The level of water in the U-shaped delivery tube dipped in water of the beaker rises after sometime.   

EXPERIMENT TO SHOW THAT CO2 IS GIVEN OUT DURING RESPIRATION  - Teachoo.jpg

        

Result:

  • CO 2 is released by the germinating seeds during respiration in a conical flask. 
  • This CO 2 is absorbed by KOH which is hanging inside the conical flask. 
  • Due to absorption of CO 2 , a vacuum is created which causes an upward movement of water present inside the delivery tube which can be seen by change in level of water in that tube. 

 

Precautions:

  1. Germinating seeds should be kept moist.
  2. All connections of the set-up should be air-tight.
  3. Freshly prepared KOH solution should be used.
  4. Keep one end of the U-shaped delivery tube in a conical flask and the other end immersed in water of the beaker.
  5. The test tube containing KOH should be hung carefully.

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CA Maninder Singh is a Chartered Accountant for the past 12 years and a teacher from the past 16 years. He teaches Science, Accounts and English at Teachoo