Sohan designed an experiment for the regeneration of sugarcane, where he used cuttings to grow sugarcane. He used two types of cuttings, type ‘A’ and type ‘B’ (Fig. 3.23). After a few weeks, type ‘B’ cuttings sprouted and developed into sugarcane plants, whereas the type ‘A’ cuttings did not sprout.
(i) Why were the type ‘B’ cuttings able to grow as sugarcane but type ‘A’ could not?
(ii) What difference was present in type ‘B’ compared to type ‘A’?
(iii) What observation or measurement was made to determine whether this change had an effect?
(iv) What parameters should be kept the same for both types of cuttings to ensure a fair comparison?
Question 10 — Sohan designed an - Tissues in Action - [Teachoo] - Questions at the end of the chapter
Cutting A — no node: does not sprout X
Cutting B — has a node with a bud: sprouts /
Node contains meristematic tissue
Keep soil, water, light the same (fair test)
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(i) Type B grew because its cutting included a node with an intercalary or apical meristem, which can divide and form new shoots; type A had no such growing region.

(ii) Type B contained a node (with buds and meristematic tissue), while type A was a piece of internode without one.

(iii) The observation was that type B sprouted and grew into sugarcane plants while type A did not.

(iv) Soil, water, light, temperature and the size of the cuttings should be kept the same, so only the presence of a node differs.

Sugarcane Cuttings A and B
Type B had a node
The node carries buds and meristematic tissue.
Type A had no node
No dividing region, so no new shoot.
Only type B sprouted into a plant
Back to: 3.2.3 Intercalary meristem — How do plants grow after being cut?
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