CLASS XII (2017-18)

One Paper                                                                       Max. Marks 70+30

                                                                                                    Time: 3 hrs.

Units    No. of periods  Marks
Unit - V Protein and Gene Manipulation  100 40
Unit - VI Cell Culture and Genetic Manipulation  80 30
  Practicals 60 30
  Total  240 100


One paper                                                               Time: 3 hrs.

                                                Total Marks: 70       180 Periods
Unit-V Protein and Gene Manipulation                                 40 Marks           100 Periods

Chapter-1: Recombinant DNA Technology

Introduction, Tools of Recombinant DNA technology, Making Recombinant DNA, Introduction of Recombinant DNA into Host Cells, Identification of recombinants, Polymerase chain reaction (PCR), Hybridization techniques, DNA library, DNA sequencing, Site-directed mutagenesis

Chapter-2: Protein Structure and Engineering

Introduction to the World of Proteins, 3-D Shape of Proteins, Structure-function relationship in Proteins, Purification of proteins, Characterization of proteins, Protein Based products, Designing proteins (protein engineering

Chapter-3: Genomics, Proteomics and Bioinformatics

Introduction, Genome sequencing projects, Gene prediction and Counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, History of bioinformatics, Sequences and Nomenclature, Information sources, Analysis using bioinformatics Tools

Unit-VI Cell Culture and Genetic Manipulation                30 Marks                 80 Periods

Chapter-1: Microbial Cell Culture and Its Applications

Introduction, Microbial culture techniques, Measurement and kinetics of microbial growth, Scale-up of microbial process, Isolation of microbial products, Strain isolation, improvement and Preservation, Applications of microbial culture technology, Biosafety Issues in microbial technology

Chapter-2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Gene transfer methods in plants, Transgenic plants with beneficial traits, Biosafety of Transgenic Plants.

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Characterisation of cell lines, Methods of gene delivery into cells, Scale-up of animal culture process, Applications of animal cell culture, Stem cell technology, Tissue engineering


PRACTICALS                          30 Marks                         60 Periods

Note: Every student will be required to do the following experiments during the academic session.

List of Experiments

1. Use of special equipment in biotechnology experiments.

2. Isolation of bacterial plasmid DNA

3. Detection of DNA by gel electrophoreses

4. Isoloation of Genomic DNA (CTAB method)

5. Estimation of DNA

6. Bacterial transformation using any plasmid

7. Restriction digestion of plasmid DNA & its analysis by gel electrophoreses

8. Isolation of bacterial grom curd & staining of bacteria

9. Cell viability assay

10. Data retreival and data base search using internet site NCBI and download a DNA and protein sequence from internet, analyse       it and comment on it

11. Reading of a DNA sequencing gel to arrive at the sequence

12. Project work


Scheme of Evaluation:

Time: 3 Hours                                                          Max. Marks 30

The scheme of evaluation at the end of the session will be as under:


A Two experiments 6+6 (only one computer based practicals
  Practical record 4
  Viva on Practicals 4
B Project work  
  Write up 5
  Viva on Projects 6
  Total  30 Typology of Questions Very Short Answer (VSA) 1 marks Short Answer-I (SA-1)2 marks Short Answer-II (SA-2)3 marks Long Answer (L.A) (5 marks) Total marks % Weightage
1 Knowledge Based  2 1 2 1 15 22%
2 Conceptual Understanding (Application and Reasoning based) 3 4 8 1 40 57%
3 Higher Order Thinking Skills (HOTS)  1 2                                                            -                                                          - 5 7%
4 Skill Based                                                                   - 1 1 1 10 14%
  Total  6 8 11 3 70 100%


About the author
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Ritu Sagar